Radioimmunoassay of Thyroxine by Thyroscreen
نویسندگان
چکیده
منابع مشابه
Determination of free thyroxine in serum by radioimmunoassay.
We describe a sensitive and specific radioimmunoassay for free thyroxine, directly determined in the diffusate obtained from equilibrium dialysis of serum. The intra- and interassay precisions were 7.5 +/- 0.27 and 9.1 +/- 0.58 ng/liter (mean +/- SD), respectively. The free thyroxine concentrations of normal persons and patients with thyroid disease were as follows: normal, 7.6 +/- 1.7; euthyro...
متن کاملRadioimmunoassay for human thyroxine-binding prealbumin.
A radioimmunoassay (RIA) for human thyroxine-binding prealbumin (PA) is described. It employs highly purified PA, anti-human PA serum at 1:30,000 final dilution, normal bovine serum as a carrier, and polyethyleneglycol to precipitate the immune complexes. This assay is extremely sensitive (limit of detection less than 0.2 micrograms per dL or less than 3.6 X 10(-15) moles per tube), accurate (r...
متن کاملMicroencapsulated antibodies in radioimmunoassay. II. Determination of free thyroxine.
We describe a method for directly measuring free thyroxine in human serum. Antibody to thyroxine was encapsulated within a semipermeable nylon membrane that excludes substances of relative molecular mass greater than 20,000. The microencapsulated antibody was then presaturated with [125I]thyroxine. Serum incubated with the microcapsules initiated a displacement reaction between free thyroxine a...
متن کاملSimultaneous radioimmunoassay of thyrotropin and thyroxine in human serum.
The importance of early diagnois and treatment of congenital hypothyroidism has been well established, and several screening programs have been undertaken to detect neonates with this disorder by measurement of concentrations of thyrotropin or thyroxine in the serum. However, measurement of either hormone alone may fail to identify all affected patients. Accordingly, we have established a simul...
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ژورنال
عنوان ژورنال: RADIOISOTOPES
سال: 1979
ISSN: 1884-4111,0033-8303
DOI: 10.3769/radioisotopes.28.2_104